To further determine whether these mutations improve nodule cell infection, we inoculated Mimosa pudica individually with E16, K16, M5, their derivatives harboring the wild-type (WT) phc alleles, their respective nodulating ancestors CBM212, CBM349, and CBM356, and mutants of these ancestors carrying the phc mutations (M5, which is statistically as infectious as M16 [26], was used rather than M16, since genetic transformation failed in the latter clone in spite of many trials). Here, SLC25A3 is linked to infection.