FAT1 and glioblastoma: In order to confirm that the increase in migration, invasion and colony forming capacity of GBM cells mediated by FAT1 [17, 19] occurs under the transcriptional control of NFkB (RelA), we transfected U87MG cells with NFkB (RelA) specific siRNA followed by in-vitro transwell migration and invasion assays and soft agar colony formation assay.