Previous studies have reported that Rho GTP enzyme family regulated cytoskeletal changes and cell motility, including tumour cell migration and invasion, we then accordingly tested the levels of Rho GTPase family‐related protein RhoA, Rac1 and Cdc42, and key proteins involved in Rho/Rock and PI3K/AKT downstream signal pathway, including Rock, AKT, phosphorylated AKT (p‐AKT), myosin light chain (MLC) and phosphorylated myosin light chain (p‐MLC) after the overexpression or inhibition of CXCL12, CXCR4 and CXCR7 genes. The gene discussed is CXCR4; the disease is neoplasm.