Based on the expression in HCC, 63 differentially expressed genes in the TCGA dataset (logFC > 1 or logFC < −1, adjust P-value < 0.05) (Fig. 1A) were screened out, of which FOS, DIRAS3, NRG1, FOXO1 were down-regulated in HCC. This evidence concerns the gene NRG1 and hepatocellular carcinoma.