Although this study has some limitations due to the small sample size, future investigations aimed at improving our understanding of the role of the BCR repertoire in immune responses, autoimmunity and autoreactivity are anticipated as the cost of HTS decreases: first to analyze the repertoires of different B cell subsets in patients with SSc, and second to further identify the features of the BCR repertoire and the functions of specific BCR genes in different patients with SSc. The gene discussed is BCR; the disease is systemic sclerosis.