Indeed, to establish the α-synuclein RT-QuIC in the diagnostic of α-synucleinopathies (pre-mortem in CSF or post-mortem in brain) the following standardization steps will be required: 1) Pre-analytical sample treatments (type of storage tube), 2) harmonization of test-protocols (composition of the reaction mix, kind of α-synuclein substrate, incubation times and shaking etc.), 3) definition of a positive reaction, 4) determination of stability of the misfolded α-synuclein seed against storage conditions, and 5) investigation of the tolerance of the RT-QuIC against blood contamination. The gene discussed is SNCA; the disease is synucleinopathy.