Manne and co-workers [107] achieved α-synuclein aggregation in the RT-QuIC by using as seed either CSF or brain homogenates in combination of SDS and chaotropic agents (zirconia/silica beads, similarly to Fairfoul’s protocol) to enhance the conversion of monomeric α-synuclein in RT-QuIC reactions, seeded with DLB and PD brain homogenates, with PD CSF and with a subset of progressive supranuclear palsy (PSP, a neurodegenerative disease characterized by tau aggregates). The gene discussed is MAPT; the disease is Parkinson disease.