To test this, we immunized hCD155-tg mice with either mOVA2 or DMEM (Mock) by the intramuscular (i.m.)route and isolated skeletal muscle tissue from the inoculation site to assess the presence of mOVA2 by plaque assay; isolated skeletal muscle tissue from the inoculation site (24hpi) for tests of cytokine responses (Fig. 4a; compare to in vitro BMDC infection in Fig. 3c, d; Supplementary Fig. 7); assessed local immune cell infiltrates by flow cytometry (Fig. 4b); and tested DC maturation status by evaluating CD40 and CD86 expression on local infiltrating DCs (Fig. 4c). This evidence concerns the gene CD86 and infection.