Hierarchical clustering demonstrated enrichment in MYCNhigh HCC cells for genes related with liver CSC markers (EpCAM and AFP), stemness regulation (DLK1, NANOG, and ALDH1A1), and fatty acid metabolism (ACACA, FASN, FADS1, SCD1, and FADS2) (Fig. 2c). This evidence concerns the gene NANOG and hepatocellular carcinoma.