Moreover, to assess whether the accumulation of free ATG12 and the additional ATG12-containing complex is SETD2 dependent, four additional RCC cell lines, CAKI-2 and 769-P both lines characterized by VHL gene mutation but a wild-type SETD2 gene, as well as A498 and RCC-FG2 both lines characterized by VHL and SETD2 (loss of function) gene mutation were analyzed (Table 1). Here, ATG12 is linked to renal cell carcinoma.