A reduced autophagic flux, as illustrated by reduced LC3-II/LC3-I ratio upon BafA1 treatment, in the SETD2-deficient A498 and RCC-FG2 RCC cells, as compared with the CAKI-2 and 769-P RCC cells, which carry a wild-type version of this methyltransferase enzyme was also observed (Supplementary Figs. 2c, d and 3f, g). This evidence concerns the gene SETD2 and renal cell carcinoma.