Moreover, a multiple regression analysis using soluble Klotho levels as the dependent variable and age, sex, smoking, HT, DM, and serum levels of total cholesterol, phosphorus, uric acid, NLR and TNFα/IL10 ratio as covariates (Table 3B) showed that age (β=-0.234, P<0.05) and TNFα/IL10 ratio (β=-0.243, P<0.01) were significantly associated with soluble Klotho concentration in the full-study cohort (adjusted R2: 0.068, P<0.05), while only the association with the TNFα/IL10 ratio remained significant in the age-matched subgroup (adjusted R2: 0.232, P<0.05). The gene discussed is TNF; the disease is diabetes mellitus.