Furthermore, transient transfection of HCT‐15, HCT‐116, SW 620, or DLD‐1 colon cancer cells with an NF‐κB‐luciferase plasmid followed by treatment with acetylshikonin showed significant inhibition of NF‐κB transcriptional activity (Figure 4b). The gene discussed is NFKB1; the disease is malignant colon neoplasm.