Because the initial assessment of the expression of pTOPK, TOPK, and the members of TOPK signalling pathway, such as pERK, pRSK, and pc‐Jun, was elevated in HCT‐15, HCT‐116, SW 620, and DLD‐1 colon cancer cells, compared to HT‐29 and SW 480 colon cancer cells (Figure S2B), we studied the effects of acetylshikonin on HCT‐15, HCT‐116, SW 620, and DLD‐1 colon cancer cell lines. This evidence concerns the gene EIF2AK3 and colonic neoplasm.