NFKB1 and colonic neoplasm: Furthermore, transient transfection of HCT‐15, HCT‐116, SW 620, or DLD‐1 colon cancer cells with an NF‐κB‐luciferase plasmid followed by treatment with acetylshikonin showed significant inhibition of NF‐κB transcriptional activity (Figure 4b).