First, all 16 patients (described in Table III) were pre-screened for deletions and/or mutations in DPY19L2 and SPATA16, using a combination of targeted next-generation sequencing (NGS) and Sanger sequencing to detect point mutations and CNVs in these two genes and other known infertility genes (Oud et al., 2017). This evidence concerns the gene DPY19L2 and Infertility.