SPATA16 and Infertility: First, all 16 patients (described in Table III) were pre-screened for deletions and/or mutations in DPY19L2 and SPATA16, using a combination of targeted next-generation sequencing (NGS) and Sanger sequencing to detect point mutations and CNVs in these two genes and other known infertility genes (Oud et al., 2017).