In contrast with in vitro transformation, we did not observe gross changes to the balance between progenitor and differentiated cells in the resulting WT vs KO leukemias, which had preservation of phenotypic leukemia stem-like cells (LSC), defined by the Lin-Kit+Sca1-CD34+CD16/32+ GMP surface phenotype (L-GMP) (Figure 2C). The gene discussed is CD34; the disease is leukemia.