SPAG5 and ovarian carcinoma: The present study aimed to detect SPAG5 messenger RNA (mRNA) expression levels in fresh ovarian cancer samples via one-step quantitative reverse transcription-polymerase chain reaction (qPCR), detect SPAG5 protein expression by Western blot (WB), and analyze the SPAG5 protein in an ovarian cancer tissue microarray (TMA) using immunohistochemistry (IHC).