In addition, although no significant enrichment was observed for either truncating or nontruncating variants (analyzed separately) in ACTC1, NEXN, and PLN, patients with DCM of the secondary diagnostic referral cohort were significantly enriched for aggregated protein-altering variants in these 3 genes (truncating + nontruncating; ACTC1 0.47% vs 0.07%, P=1.9×10−2, NEXN 2.9% vs 0.7%, P=1.6×10−2, PLN 0.4% vs 0.04%, P=2.7×10−2). This evidence concerns the gene PLN and familial dilated cardiomyopathy.