As previously revealed data were achieved using animal model, sarcoma cell lines and molecular evaluation (Walter et al., 2014; Zhang et al., 2013; de Nigris et al., 2008; Fan et al., 2008), our study has been more focused on the protein expression levels of two CSC markers CD133 and CXCR4 using immunohistochemical detection in the human OS tissues. This evidence concerns the gene PROM1 and sarcoma.