In this study, we assessed the presence and prognostic value of CD3, CD4, CD8, FoxP3, and PD1 positive TILs, as well as the CD8/FoxP3 ratio, in the head and neck tumor epithelium in pre-treatment biopsies of HNSCC patients using an objective, digital pathology-aided method. The gene discussed is CD8A; the disease is head and neck neoplasm.