In conclusion, this study did not provide evidence for a prognostic value of the presence of CD3 + , CD4 + , CD8 + , FoxP3 + , and PD1 + T lymphocytes in the tumor epithelium of advanced stage, HPV-negative HNSCC patients treated with primary chemoradiotherapy. The gene discussed is FOXP3; the disease is head and neck squamous cell carcinoma.