We found that RNA interference (RNAi)-mediated depletion of PDZRN3 by infection with an adenovirus encoding a short hairpin RNA (shRNA) specific for PDZRN3 mRNA (KD) in C2C12 myoblasts maintained in growth medium (GM) resulted in a significant decline in cell number compared with control cells infected with an adenovirus encoding a scrambled version of the PDZRN3 shRNA sequence (Scramb) (Fig. 1a,b). Here, PDZRN3 is linked to infection.