ACTA1 and metabolic dysfunction-associated steatohepatitis: Histopathological analyses were supported by quantitative image analysis of liver lipid, collagen 1a1, α-SMA, and galactin-3 content, all being significantly upregulated in the DIO NASH model versus chow control (p < 0.001, p = 0.042, p < 0.001, p < 0.001, Fig. 1C).