Quantitative proteomics and transcriptomics both showed increased expression of the standard NASH markers like Col1a1, Acta2 (α-SMA), and Lgals3 (Galactin-3/Mac2) in DIO-NASH compared to chow controls (transcriptomics: p < 0.001, p = 0.01, p < 0.001, Fig. 2B). This evidence concerns the gene ACTA2 and metabolic dysfunction-associated steatohepatitis.