AKAP8 and malignant colon neoplasm: To generalize the findings, we examined the effect of AKAP8 knockdown in a different epithelial cell line, HCT116 colon cancer cells, and observed that knockdown of AKAP8 alone is sufficient to induce a loss of epithelial characteristics, as demonstrated by the gain of elongated spindle-like mesenchymal morphology and the decrease in expression of epithelial markers (Fig. 2d, e).