MARCKS and Alzheimer disease: The active necrosis revealed by pSer46-MARCKS were present at significantly higher frequencies in all MCI patients than in AD patients (Fig. 2d, e), and neurons surrounded with reactive pSer46-MARCKS, even though they did not match the strict criteria of active necrosis because the nuclear DAPI stain remained intact, were increased in MCI (Supplementary Fig. 7).