In our immunohistochemistry of pRIP1/3 and pMLKL, with brain samples of AD model mouse and human AD patient, we could not detect co-localization of phosphorylated RIP1/3 and phosphorylated MLKL, which is essential for signal transduction of necroptosis, in any single neuron of 5xFAD or APP-KI mice at 3 months of age or of human MCI and AD patient (Supplementary Fig. 17). This evidence concerns the gene APP and Alzheimer disease.