CD4 and synucleinopathy: We also evaluated changes in the adaptive immune system by analyzing the gene expression levels of two markers of T lymphocytes, Cd4, and Cd8. RT-qPCR analysis showed no changes between animal groups (Fig. 2c and Additional file 3: Figure S3), excluding an effect of the synucleinopathy or diet on T cell recruitment and discarding the involvement of the adaptive immune system in neuroinflammation in PLP-hαSyn mice (Fig. 2c and Additional file 3: Figure S3).