In a second paper from 2017, Yamamoto and colleagues used the hydrodynamic tail vein injection/Sleeping Beauty transposon model to overexpress myc, Yap-1, AKT, Notch1, and Notch2 alone and in combination to generate tumors resembling HB, HCC, and cholangiocarcinoma [52]. This evidence concerns the gene YAP1 and hemoglobin measurement.