Subsequently, the rescue assays of proliferation, tumor stemness, and invasion also testified that cancer-promoting effect caused by ZEB1-AS1 overexpression was saved by knocking down HOXB8 (Figure 7F–7H), indicating that ZEB1-AS1 promoted malignant progression of CCA partly by promoting HOXB8. This evidence concerns the gene ZEB1 and cholangiocarcinoma.