By detecting the differential expression of p16INK4a, Notch1, and hTERC genes, the relationship between these genes and the occurrence and development of Mongolian patients with cervical cancer was analyzed, and screen the best reference biomarkers for early diagnosis of cervical cancer in Mongolian population, and establish the relationship between these three genes and HPV16 infection. The gene discussed is NOTCH1; the disease is cervical cancer.