The positive expression rates of p16INK4a gene in cervical cancer, CIN‐III, CIN‐II, CIN‐I, uterine leiomyoma, and chronic cervicitis were 100.00% (45/45), 91.43% (32/35), 59.38% (19/32), 52.63% (20/38), 42.86% (18/42), and 26.47% (9/34), respectively. The gene discussed is CDKN2A; the disease is cervical cancer.