Other than the favorable biological functions such as inhibition of cell proliferation and migration [35, 36] and activation of the IGF1R/PI3K/AKT pathway [37], miR-31 influences the BC apoptosis by protein kinase C epsilon [38, 44] and regulates stem cell self-renewal and tumorigenesis by Wnt/β-catenin signaling [39], which is consistent with our enrichment analysis. This evidence concerns the gene AKT1 and breast cancer.