Given the emerging role for ASICs in migraine (Karsan, Gonzales, & Dussor, 2018), the expression of ASIC3 in the trigeminal ganglion (Ichikawa & Sugimoto, 2002) and the trigeminal nucleus caudalis (TNC; Wang et al., 2018) along with the enhancement of ASIC3 activity by NO donors (Cadiou et al., 2007), we sought to determine the role of ASIC3 in migraine and further determine if NO‐induced hyperalgesia may be in part ASIC3‐dependent. This evidence concerns the gene ASIC3 and migraine disorder.