The constructs were then transiently transfected into mouse neuroblastoma Neuro2a cells for 48 h before co-immunostaining for the Myc tag and RCAS1 (Golgi marker) or PDI (Endoplasmic Reticulum marker) or POMC (neuropeptide vesicle marker) (Fig. 7b). Here, EBAG9 is linked to neuroblastoma.