FOXA1 and Familial prostate cancer: In accordance with data from our prostate cancer cell clones stably expressing wild-type Cas9 and dCas9-KRAB, individual CRE deletion resulted in a significant reduction in FOXA1 mRNA expression: (ΔCRE1 ~ 29.3 ± 7.3%, ΔCRE2 ~ 36 ± 11.8%, ΔCRE3 ~ 30.6 ± 12.7%, ΔCRE4 ~ 24.5 ± 6.1%, ΔCRE5 ~ 23.7 ± 13.2%, ΔCRE6 ~ 26.8 ± 14.2% and ΔFOXA1 ~ 96.2 ± 1.4% reduction (Student’s t test, p < 0.05, Fig. 5a, Supplementary Fig. 8a–f).