AFP and hepatocellular carcinoma: To demonstrate the universal expression of circRNA-100,338 in HCC cell lines, we selected normal liver cell line L02, AFP-positive, AFP-negative, highly metastatic potential and lowly metastatic potential HCC cell lines, including HLE, Huh7, Hep3B, BEL7402, SMMC7721, MHCC97L, MHCC97H, HCCLM3, and HCCLM6.