In this study, we confirmed in CR-CSCs our results previously obtained in immortalized cancer cell lines (HT29 and CaCO2) [17]: A relationship exists between differentiation and the increase of sEVs release which sustains the hypothesis that cells adopt sEVs to get rid of molecules, such as CSC markers CD133 (Figure 3b), and to send specific signals to surrounding cells. The gene discussed is PROM1; the disease is cancer.