CD147 knock-down in HT29 cancer cells induced a two-fold decrease of whole Calcein-AM positive extracellular vesicles (20–1000 nm in size), three-fold decrease of vesicles with size from 150 to 200 nm (mEVs) and four-fold decrease of sEVs (<150 nm in size) compared to mock-transfected cells (Figure 7). Here, BSG is linked to cancer.