For instance, ankaflavin, a traditional food additive used in Eastern Asia and China, significantly decreased the proliferation of Kupffer cells and the protein expression of inflammatory cytokines (tumor necrosis factor α (TNF-α), interleukin (IL)-6, and IL-1β) and reduced apoptosis and liver steatosis in high-fat-diet-fed mice [58]. Here, TNF is linked to fatty liver disease.