TUSC2 and thyroid cancer: Finally, in order to verify the results obtained for the role of TUSC2 in the apoptotic process of thyroid cancer cells, a trypan blue assay was performed in Nthy-ori 3-1 cells transfected with siRNAs targeting TUSC2 (siRNA TUSC2-5, siRNA TUSC2-6) or with Control siRNA and treated with doxorubicin and staurosporine.