TARDBP and amyotrophic lateral sclerosis: In particular, they used subcellular fractionation and FACS to isolate diseased neuronal nuclei with depleted TDP-43 (TDPneg nuclei) from the post-mortem brain samples of frontotemporal degeneration-amyotrophic lateral sclerosis (FTD–ALS) patients (Liu et al., 2019).