MLANA and melanoma: To explore the impact of TCR/CD8-binding avidity for pMHC on different vaccine-induced T-cell responses according to peptide and CpG doses, we generated large representative libraries of Melan-A-specific CD8 T-cell clones (n = 454) derived from effector-memory CD28pos (early-differentiated EM28pos) or CD28neg (late-differentiated EM28neg) cells by direct ex vivo sorting and cloning from seven melanoma patients (Figure 1E, Supplementary Figure 3).