IFNG and melanoma: To validate CIITA as target of the seven considered miRNAs not only in macrophages but also in different cells, and considering the low transfection efficiency of macrophages, we adopted two different cell models: a melanoma cell line (M121224) stimulated with IFN-γ (24) and HeLa cells stably expressing CIITA (HeLa-CIITA) (32).