Immunohistochemical (IHC) results showed that TNAP and α-SMA were less expressed in the heart of patients after 1 day of MI, whereas both TNAP and α-SMA were upregulated in the border zone of the heart combined with collagen deposition in patients with previous MI (Fig. 1e). The gene discussed is ACTA1; the disease is myocardial infarction.