Importantly, the positive effect of de-methylation on transcriptional activity for some immune-related genes, including PD-L1 and genes of the interferon signaling cascade, has been validated in vitro, corroborating that epigenetic modulation might be a useful tool to sensitize patients to anti-PD-L1 ICB, and the facilitation of tumor escape by repressing IFN regulatory factor 8 transcriptional expression [185]. Here, CD274 is linked to neoplasm.