The most distinct pattern of Aggregatin immunostaining observed in AD was that Aggregatin was remarkably immunoreactive within the center of amyloid plaques, which were stained by the pan-Aβ antibodies 6E10 and 4G8, the N-terminal truncated and modified pyroglutamate Aβ species Aβ[N3pe] antibody 82E1, fibrillar Aβ dye thioflavin-S (Thio-S) or oligomer Aβ antibody NU-425 (Fig. 2a, b and Supplementary Fig. 6a–e). Here, FAM222A is linked to amyloidosis.