The results showed that genes with significant 5hmC gain or loss in the AMI group were mainly enriched in apoptosis, vascular smooth muscle contraction, VEGF signaling pathway, platelet activation, HIF-1 signaling pathway, cardiac muscle contraction, and metabolic pathways, which are closely associated with acute myocardial infarction (Additional file 4: Figure S3C and S3D). This evidence concerns the gene VEGFA and acute myocardial infarction.