We validated that the corresponding APP, CD44 and CLSTN1 exons were frequently skipped, while the sixteenth MBNL1 exon and the tenth ZNF207 exon were retained following knockdown of either LUCAT1 or PTBP1 in CRC cells, both by qPCR and RT-PCR analyses using specific designed primers (Fig. 4e, f and Additional file 1: Figure S8B-D). The gene discussed is LUCAT1; the disease is colorectal carcinoma.