Moreover, in mouse tumors,the expression of BRF2 was markedly decreased in miR-425-5p tumors than that in A549 andA549-NC tumors (Figure 4C),indicating that miR-425-5p also negatively regulated BRF2 expression invivo. Especially, Spearman rank correlation test further proved thesignificantly negative correlation between miR-425-5p expression and BRF2 expression inmouse tumor tissues (Figure 4D).In summary, our results indicate that miR-425-5p inhibits the growth of A549 cells bothin vitro and in vivo through suppressing BRF2expression. This evidence concerns the gene BRF2 and neoplasm.