In this study, we uncovered the expression and function of microRNA-425-5p in patients withNSCLC and NSCLC cell line A549 and identified miR-425-5p as a novel NSCLC suppressor.Bioinformatic analysis and luciferase reporter assay showed that BRF2 was a direct targetgene of miR-425-5p. This evidence concerns the gene BRF2 and non-small cell lung carcinoma.