Furthermore, previous studies have revealed that HFD constitutes a well-established model for the induction of insulin resistance in both the periphery and brain [57] because the brain of HFD-fed mice show increased IRS-1 phosphorylation at Ser307 and inhibited IRS-1 phosphorylation at Tyr608 in comparison with the brains of control mice [80]. This evidence concerns the gene IRS1 and Insulin resistance.