In this work, we show that, for triple negative breast cancer (TNBC), an autophagy-dependent type of cancer, breast cancer cells treated with chloroquine (CQ), one of the drugs most commonly used to inhibit autophagy in cancer clinical trials, or with the genetic inhibition of autophagy, increased reactive oxygen species (ROS) production, probably due to damaged mitochondria accumulation, and secreted macrophage migration inhibitory factor (MIF) to the culture media. Here, MIF is linked to breast cancer.