In this study we have complemented the previously published biological profiles of these analogs [7], analyzing their anticancer potential on colorectal cancer (CRC) cell lines as well as their CYP24A1-dependent metabolism, compared to 1,25-dihydroxycholecalciferol (1,25-dihydroxyvitamin D3, 1,25D3) as a gold standard. The gene discussed is CYP24A1; the disease is colorectal cancer.