Knocking down mIGF1 via the CRISPR/Cas9 system confirmed that the levels of iNOS, TNF-α, and PP65 are increased in the lumbar spinal cord of ALS mice Taken together, these data indicated that IGF1-mediated suppression of NF-κB activation in microglia is a novel molecular mechanism that underlie MN death in ALS. Here, NOS2 is linked to amyotrophic lateral sclerosis.