The resulting iDCs were either infected with HSV-2 strain G or HSV-1 as control, and harvested at 2, 4, 8, or 16 hpi or matured into mDCs, via stimulation with a maturation cocktail, additionally containing IL-1β, IL-6, TNF-α, and PGE2, followed by the same infection procedure as for iDCs. Here, TNF is linked to infection.