A total of fourteen DMGs were discovered in our integrated analysis, eleven of which (KLK13, PRSS27, COL5A2, KRT4, MFAP2, SCNN1B, SIX4, CRABP2, IL1RN, EHD3 and GPX3) were associated with prognosis of ESCC and also exhibited a negative correlation between DNA methylation and mRNA expression. The gene discussed is PRSS27; the disease is esophageal squamous cell carcinoma.