A total of fourteen DMGs were discovered in our integrated analysis, eleven of which (KLK13, PRSS27, COL5A2, KRT4, MFAP2, SCNN1B, SIX4, CRABP2, IL1RN, EHD3 and GPX3) were associated with prognosis of ESCC and also exhibited a negative correlation between DNA methylation and mRNA expression. Here, KRT4 is linked to esophageal squamous cell carcinoma.