Moreover, it was indicated by Western blot that silencing of circ‐TFF1 induced a decline in the expression of TFF1, Ki67, Bcl‐2, MMP2, MMP9 and N‐cadherin, but an increase in the levels of Bax and E‐cadherin (Figure 7E), further confirming the inhibitory effects of circ‐TFF1 depletion on cell proliferation, metastasis and EMT in breast cancer. This evidence concerns the gene MMP9 and breast cancer.