The functional significance of nuclear Atox1 was examined first in SW620 cells where knock-down of Atox1 expression resulted in decreased cell migration and colony formation which was unaffected by activin A. Further, we demonstrate that Atox1 knockdown by siRNA induced the most prominent proliferation suppression in metastatic SW620 colon cancer cell lines. This evidence concerns the gene ATOX1 and colonic neoplasm.