The functional significance of nuclear Atox1 was examined first in SW620 cells where knock-down of Atox1 expression resulted in decreased cell migration and colony formation which was unaffected by activin A. Further, we demonstrate that Atox1 knockdown by siRNA induced the most prominent proliferation suppression in metastatic SW620 colon cancer cell lines. The gene discussed is ATOX1; the disease is malignant colon neoplasm.